Isolation of the ARO1 cluster gene of Saccharomyces cerevisiae.
نویسندگان
چکیده
منابع مشابه
Isolation, Subtype Determination, Cloning and Expression of HBsAg Gene from an Iranian Carrier in Saccharomyces cerevisiae
The Hepatitis B Surface antigen ( HBsAg) gene was isolated from an Iranian HBeAg positive carrier by PCR. The gene was cloned in pUC19 for sequencing and pYES2 for expression in Saccharomyces cerevisiae, which pNF1 and pDF3 constructs were made respectively. The sequencing data showed that the isolated HBsAg gene shared more than 90% homology with the ayw subtype. The pDF3 was transferred into ...
متن کاملIsolation of indigenous Glutathione producing Saccharomyces cerevisiae strains
Background: Glutathione (GSH) is a non-protein thiol compound, which plays an important role in the response to oxidative stress and nutritional stress. The aim of this study was to isolate indigenous S. cerevisiae strains capable of effectively produce GSH. Methods: One hundred-twenty sweet frui...
متن کاملCLONING AND EXPRESSION OF HUMAN IFNα2B GENE IN SACCHAROMYCES CEREVISIAE
Interferon is a protein secreted by eucaryotic cells following stimulation by viruses, bacteria, and many other immunogenes. Recent medical studies indicate that interferons have effective role in the treatment of virus infections, immunodeficiency and certain types of cancer such as hairy cell leukaemia (HCL). The aim of the present study is to apply yeast strain for secreting human IFNα2b fol...
متن کاملisolation of high ethanol resistant strains of saccharomyces cerevisiae
several strains of saccharomyces cerevisiae were isolated from different sources. these strains were under taken for ethanol tolerance analysis and growth under stress condition. exponential phase to various concentrations of ethanol (2–26% v/v) for 1 h was used for isolation of resistant strains. viable cells were isolated and purified by inoculation of diluted samples on yeast extract peptone...
متن کاملIsolation and Characterization of Saccharomyces cerevisiae Mutants
Aculeacin A is a lipopeptide that inhibits ,B-glucan synthesis in yeasts. A number of Saccharomyces cerevisiae mutants resistant to this antibiotic were isolated, and four loci (ACRI, ACR2, ACR3, and ACR4) whose products are involved in the sensitivity to aculeacin A of yeast ceils were defined. Mutants containing mutations in the four loci were also resistant to echinocandin B, another member ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1983
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.3.9.1609